Image analysis for the automated estimation of clonal growth and its application to the growth of smooth muscle cells |
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Authors: | V. C. Gavino G. E. Milo D. G. Cornwell |
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Affiliation: | Department of Physiological Chemistry, The Ohio State University, U.S.A. |
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Abstract: | Abstract. Image analysis was used for the automated measurement of colony frequency ( f ) and colony diameter ( d ) in cultures of smooth muscle cells, Initial studies with the inverted microscope showed that number of cells ( N ) in a colony varied directly with d : log N = 1.98 log d - 3.469 Image analysis generated the complement of a cumulative distribution for f as a function of d . The number of cells in each segment of the distribution function was calculated by multiplying f and the average N for the segment. These data were displayed as a cumulative distribution function. The total number of colonies ( fT ) and the total number of cells ( NT ) were used to calculate the average colony size ( NA ). Population doublings (PD) were then expressed as log2 NA . Image analysis confirmed previous studies in which colonies were sized and counted with an inverted microscope. Thus, image analysis is a rapid and automated technique for the measurement of clonal growth. |
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