短途运输应激对Wistar大鼠的影响

目的研究短途运输应激对Wistar大鼠的影响,建立评价啮齿类实验动物短途运输应激的技术指标体系。方法采用Wistar大鼠进行短途运输实验,测定其在运输中及运输后各阶段的代谢、神经内分泌和免疫功能主要指标,与未经历运输的对照组比较,分析短途运输应激的影响。结果Wistar大鼠血糖（GLU）在运输0.5 h和1 h时均升高,而在运输1.5 h时降低,运输结束后24 h时GLU再次升高;血清皮质酮（CORT）亦在运输0.5 h和1h时升高而运输1.5 h时降低,但运输结束24 h起即恢复正常;血清β-内啡肽（-βEP）从运输起至运输结束24 h时均降低,至48 h恢复正常。外周白细胞总数（WBC）自运输起急剧减少,于结束运输后的72 h恢复;免疫球蛋白G（IgG）、白介素2（IL-2）、γ干扰素（IFN-γ）及白介素1（IL-1）的血清水平在运输中及运输结束的即刻均没有显著变化,但在结束运输后的48 h内均不同程度降低,至72 h恢复至正常水平。肝脏hsp72 mRNA的表达随着运输时间的延长而极显著上调,运输结束后逐渐恢复,至72 h恢复至正常水平。结论短途运输应激对Wistar大鼠的代谢、神经内分泌和免疫功能均有不利影响,其经历常规短途运输（1.5 h以内）到达目的地后的健康适应期至少应为72 h;外周血中白细胞总数（WBC）、血糖（GLU）、皮质酮（CORT）、β-内啡肽（-βEP）、白介素2（IL-2）的水平可用于系统评价短途运输应激。

Influence of Stress on Wistar Rats due to Short-Term Transportation

Objective To study the possible influence of short-term transportation on Wistar rats,and to establish a technical index system to evaluate the influence of stress on laboratory rodents.Methods Wistar rats were used in the short-term transportation experiment.Main indexes of metabolism,neuroendocrine and immunity were tested. All the indexes of rats experiencing transportation were compared with those of the control group to analyze the impact of short-term transportation on laboratory rats.Results GLU of Wistar rats increased both at the moment of 0.5 h and 1 h during transportation,and at 24 h after stop of transportation,while decreased at the time of 1.5 h during transportation.Serum CORT also increased at the moment of 0.5 h and 1 h during transportation,then decreased at the moment of 1.5 h during transportation,but resumed to normal since 24 h after stop of transportation.Serum β-EP decreased from the beginning of transportation to 24 h after transportation,it did not resume to normal until 48 h after transportation.Total WBC in peripheral blood decreased sharply at all three different time points（0.5 h,1 h and 1.5 h） of transportation,and resumed at 72 h after transportation.Serum levels of IgG,IL-2,IFN-γ and IL-1 decreased in 48 h after the end of transportation though there was no significant change seen during transportation period,and resumed at 72 h after transportation.The expression of hsp72 mRNA in liver increased sharply at all the time （0.5 h,1 h and 1.5 h） of transportation,the longer the transportation period,the higher the expression of hsp72 mRNA in liver,while it resumed quickly within 72 h after end of transportation.Conclusion Short-term transportation has considerable negative impact on the metabolism,neuroendocrine and immunity in Wistar rats.Those experienced short-term transportation（1.5 h） need at least 72 h of acclimatization after the end of transportation.Parameters of total WBC in peripheral blood,GLU,CORT,β-EP,and IL-2 in serum can be used as indexes to evaluate the stress due to short-term transportation.