Advanced glycation end-product (AGE) induces apoptosis in human retinal ARPE-19 cells via promoting mitochondrial dysfunction and activating the Fas-FasL signaling |
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Authors: | Pu Wang Changzheng Chen Zhen Chen Zhimin Qian |
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Affiliation: | 1. Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan, People’s Republic of China;2. Department of Ophthalmology, Inner Mongolia People’s Hospital, Hohhot, People’s Republic of China;3. Department of Ophthalmology, Inner Mongolia People’s Hospital, Hohhot, People’s Republic of China |
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Abstract: | Advanced glycation end-products (AGEs) are extremely accumulated in the retinal vascular and epithelial cells of diabetes mellitus (DM) patients, particularly with diabetic retinopathy (DR). To elucidate the pathogenesis of the AGE-induced toxicity to retinal epithelial cells, we investigated the role of Fas–Fas ligand (FasL) signaling and mitochondrial dysfunction in the AGE-induced apoptosis. Results demonstrated that the AGE-BSA- induced apoptosis of retinal ARPE-19 cells. And the AGE-BSA treatment caused mitochondrial dysfunction, via deregulating the B-cell lymphoma 2 (Bcl-2) signaling. Moreover, the Fas/FasL and its downstreamer Caspase 8 were promoted by the AGE-BSA treatment, and the exogenous α-Fas exacerbated the activation of Caspase 3/8. On the other side, the siRNA-mediated knockdown of Fas/FasL inhibited the AGE-BSA-induced apoptosis. Taken together, we confirmed the activation of Fas–FasL signaling and of mitochondrial dysfunction in the AGE-BSA-promoted apoptosis in retinal ARPE-19 cells, implying the important role of Fas–FasL signaling in the DR in DM. |
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Keywords: | advanced glycation end-product (AGE) apoptosis human retinal cells mitochondrial dysfunction Fas–FasL |
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