Tunicamycin-induced inhibition of protein secretion into culture medium of Arabidopsis T87 suspension cells through mRNA degradation on the endoplasmic reticulum |
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| Authors: | Yuji Iwata Noriko Hayashi Kazuki Tabara Kei-ichiro Mishiba |
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| Institution: | Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan |
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| Abstract: | The N-glycosylation inhibitor tunicamycin triggers endoplasmic reticulum stress response and inhibits efficient protein secretion in eukaryotes. Using Arabidopsis suspension cells, we showed that the reduced secretion of mannose-binding lectin 1 (MBL1) protein by tunicamycin is accompanied by a significant decrease in MBL1 mRNA, suggesting that mRNA destabilization is the major cause of the inhibition of protein secretion in plants. |
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| Keywords: | Arabidopsis suspension cells tunicamycin mRNA degradation protein secretion endoplasmic reticulum |
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