Quantitation of fumonisins in corn by HPLC with o-phthalaldehyde postcolumn derivatization and their identification by LC/MS |
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| Authors: | Akiyama Hiroshi Uraroongroj Mayuree Miyahara Makoto Goda Yukihiro Toyoda Masatake |
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| Affiliation: | (1) National Institute of Health Sciences, 1-18-1, Kamiyoga, Setagaya, Tokyo 158, Japan;(2) Department of Medical Sciences, 693, Bumrungmueng Rd., Yod-se Bangkok, 10100, Thailand |
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| Abstract: | Fumonisins B1(FB1) and B2(FB2) were isocratically separated on a fluorocarbon column without using an ion pair reagent and nonvolatile buffer during the HPLC and were detected by an o-phthalaldehyde postcolumn derivatization system using a fluorescence detector. The minimum detectable concentrations of FB1 and FB2 in corn by this system were 0.01 μg/g and 0.01 μg/g, respectively. The separated fumonisins were further identified by a directly interfaced ion trap MS using electrospray ionization. FB1 and FB2 in naturally contaminated corn were identified in the selective ion monitoring mode at concentrations of 3.75μg/g and 1.44 μg/g, respectively. This revised version was published online in August 2006 with corrections to the Cover Date. |
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| Keywords: | Fumonisins o-phthalaldehyde HPLC postcolumn derivatization LC/MS |
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