Enzymatic properties of lipase and characteristics production byLactobacillus delbrueckii subsp.bulgaricus |
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| Authors: | M. M. A. El-Sawah A. A. Sherief Samia M. Bayoumy |
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| Affiliation: | (1) Microbiology Department, Faculty of Agriculture, Mansoura University, Mansoura, Egypt;(2) Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt |
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| Abstract: | Some properties of an extracellular lipase produced byLactobacillus delbrueckii subsp.bulgaricus were studied. Maximum enzyme activity was found against olive and butter oil as enzyme substrates. Addition of 9% acacia gum, 0.1% Na-deoxycholate and 0.01 M CaCl2 to the enzyme reaction mixture increased-lipase activity from 5.3 to 14.5 (FFA/mg protein/minute) at pH 6.0 and at 40° C. Maximum lipase production was reached in the presence of glucose as a sole source of carbon, wheat bran as nitrogen source, olive oil as a sole lipid source and butyric acid as fatty acid supporting the growth medium. An initial pH value of the culture medium of 6.0 and a temperature of 35° C gave the highest lipolytic activity. |
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| Keywords: | Lactobacillus delbrueckii subsp.bulgaricus lipase |
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