Selective inhibitors of KCl cotransport in human red cells |
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Authors: | J C Ellory A C Hall S O Ody H C Englert D Mania H J Lang |
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Affiliation: | Department of Physiology, University of Oxford, UK. |
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Abstract: | Two analogues of the loop diuretics furosemide and bumetanide have been identified as differential inhibitors of KCl and NaKCl cotransport systems, assayed by measuring K+ influx in 'young' human red cells. H25 inhibited both NaKCl and KCl cotransport, with I50% values of 0.03 and 30 microM respectively; H74 had no effect on NaKCl cotransport, even at 0.3 mM, but inhibited KCl cotransport with an I50% of 75 microM. These compounds are therefore useful for resolving the two transport systems. |
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