Selection of aptamers against KMP-11 using colloidal gold during the SELEX process |
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Authors: | Moreno Miguel Rincón Esther Piñeiro David Fernández Gerónimo Domingo Alberto Jiménez-Ruíz Antonio Salinas Matilde González Víctor M |
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Affiliation: | Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain. |
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Abstract: | SELEX procedure is a methodology in which single stranded oligonucleotides are selected from a wide variety of sequences based on their interaction with a target molecule. We have designed a novel SELEX methodology using colloidal gold to select high affinity single stranded DNA aptamers against Leishmania infantum KMP-11. Kinetoplastid membrane protein-11 (KMP-11) is a major component of the cell membrane of kinetoplastid parasites. Although its function is not known, the fact that KMP-11 is a cytoskeleton-associated protein suggests that it may be involved in mobility or in some other aspects of the flagellar structure. We have isolated a single stranded DNA aptamer population that binds specifically to L. infantum KMP-11. This population has been characterized in a series of in vitro experiments suggesting that it may be used as a powerful tool to further investigate the role of KMP-11 during Leishmania development and/or as a diagnostic tool in Leishmania infection. |
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Keywords: | Aptamer ssDNA Colloidal-gold KMP-11 SELEX |
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