|
|||||
|
|
| 江浙地区杨梅主要品种的ISSR分析 | |
| 引用本文： | 钱剑林,俞文生,王化坤,娄晓鸣,章镇. 江浙地区杨梅主要品种的ISSR分析[J]. 植物资源与环境学报, 2006, 15(3): 17-20 |
| 作者姓名： | 钱剑林 俞文生 王化坤 娄晓鸣 章镇 |
| 作者单位： | 1. 苏州农业职业技术学院,江苏,苏州,215008;南京农业大学园艺学院,江苏,南京,210095 2. 江苏省太湖常绿果树技术推广中心,江苏,苏州,215107 3. 南京农业大学园艺学院,江苏,南京,210095;江苏省太湖常绿果树技术推广中心,江苏,苏州,215107 4. 南京农业大学园艺学院,江苏,南京,210095 |
| 摘 要： | 为研究江浙地区杨梅（Myrica rubra Sieb．et Zucc．）的亲缘关系，运用ISSR—PCR分析方法对其主要栽培品种进行了分析。8条引物扩增出58个DNA片段，其中多态性片段42个，占总扩增片段的72．4％。结合遗传距离分析，运用UPGMA法构建了分子系统树。结果表明，所分析的14个品种和1个优良单株以0．14为结合线可分为5组，说明江浙地区的杨梅在广泛基因交流的情况下，存在局部的隔离。‘小叶细蒂’与‘极早熟1号’亲缘关系较近，前者可能是后者的亲本之一。 |
| 关 键 词： | 杨梅 品种鉴定 亲缘关系 |
| 文章编号： | 1004-0978（2006）03-0017-04 |
| 收稿时间： | 2006-03-10 |
| 修稿时间： | 2006-03-10 |
Analysis on ISSR marker of primary cultivars of waxberry in Jiangsu and Zhejiang |
|
| QIAN Jian-lin,YU Wen-sheng,WANG Hua-kun,LOU Xiao-ming,ZHANG Zhen. Analysis on ISSR marker of primary cultivars of waxberry in Jiangsu and Zhejiang[J]. Journal of Plant Resources and Environment, 2006, 15(3): 17-20 | |
| Authors: | QIAN Jian-lin YU Wen-sheng WANG Hua-kun LOU Xiao-ming ZHANG Zhen |
| Affiliation: | 1. Suzhou Polytechnical Institute of Agriculture, Suzhou 215008, China; 2. College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; 3. Extension Center for Evergreen Fruit of Taihu, Suzhou 215107, China |
| Abstract: | Inter-simple sequence repeat(ISSR) was used to analysis genomic DNA variations and genetic relationships of waxberry(Myrica rubra Sieb.et Zucc.) in Jiangsu and Zhejiang provinces.8 of 11 ISSR primers were proved to be polymorphic and informative.Total 58 DNA fragments were amplified and 42 were polymorphic(72.5%).The genetic distance was analysed according ISSR result and a dendrogram was constructed using UPGMA method.All cultivars tested were classified into 5 groups and the excellent individual(Jizaoshu No.1) has a close relationship with 'Xiaoyexidi'.This indicates that there were a wide gene flow and topical isolation between waxberry cultivars of two provinces.Maybe 'Xiaoyexidi' was a parent of 'Jizaoshu No.1'. |
| Keywords: | ISSR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |