M-PCR: a powerful method for rapid molecular identification of Trichogramma wasps (Hymenoptera: Trichogrammatidae) |
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作者姓名: | XIANG-LIDANG SHUO-YANGWEN XIAO-FANGHE XIONG-FEIPANG |
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作者单位: | LaboratoryofGeneDiversity,DepartmentofEntomology,SouthChinaAgriculturalUniversity,Guangzhou,China |
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摘 要: | Two species-specific primers were designed depending on ITS2 sequence variation of 37 Trichogramma wasps, and these primers were applied to establish an assay,multiplex PCR (M-PCR), for molecular diagnosis of two important Trichogramma wasps,T. confusum and T. dendrolimi, in China. Multiplex-PCR results showed that only target species produced two PCR products, one product of ITS2 region species-specific amplification and one product of its ITS 1 region universal amplification, but other species produced only one ITS1 universal PCR product. Using this method, the target Trichogramma species can be distinguished from other Trichogramma species. Molecular identification based on M-PCR has particular value over morphological technology and other approaches, such as normal molecular and biochemical methods. Furthermore, because M-PCR assay can avoid false negative results, which frequently happen in PCR reaction, this method will be much more accurate and useful for Trichogramma identification, and can be developed as an easy and rapid diagnostic kit applied in the identification and quality monitoring of Trichogramma mass products both in the factory and in the field. Such an easy and rapid diagnostic kit will be valuable in the application of Trichogramma species as a biological control.
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关 键 词: | 膜翅目 纹翅卵蜂科 分子鉴定 PCR Trichogramma |
M-PCR: a powerful method for rapid molecular identification of Trichogramma wasps (Hymenoptera: Trichogrammatidae) |
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Authors: | XIANG-LI DANG SHUO-YANG WEN XIAO-FANG HE XIONG-FEI PANG |
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Institution: | Laboratory of Gene Diversity, Department of Entomology, South China Agricultural University, Guangzhou, China |
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Abstract: | Abstract Two species-specific primers were designed depending on ITS2 sequence variation of 37 Trichogramma wasps, and these primers were applied to establish an assay, multiplex PCR (M-PCR), for molecular diagnosis of two important Trichogramma wasps, T. confusum and T. dendrolimi , in China. Multiplex-PCR results showed that only target species produced two PCR products, one product of ITS2 region species-specific amplification and one product of its ITS1 region universal amplification, but other species produced only one ITS1 universal PCR product. Using this method, the target Trichogramma species can be distinguished from other Trichogramma species. Molecular identification based on M-PCR has particular value over morphological technology and other approaches, such as normal molecular and biochemical methods. Furthermore, because M-PCR assay can avoid false negative results, which frequently happen in PCR reaction, this method will be much more accurate and useful for Trichogramma identification, and can be developed as an easy and rapid diagnostic kit applied in the identification and quality monitoring of Trichogramma mass products both in the factory and in the field. Such an easy and rapid diagnostic kit will be valuable in the application of Trichogramma species as a biological control. |
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Keywords: | Trichogramma species-specific primer multiplex PCR molecular identification |
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