Plant Regeneration Through Somatic Embryogenesis in Leaf Derived Callus of Plumbago Rosea |
| |
Authors: | G Das GR Rout |
| |
Institution: | (1) Plant Biotechnology Division, Plant Tissue Culture Laboratory, Regional Plant Resource Centre, Bhubaneswar, 751 015, Orissa, India |
| |
Abstract: | Regeneration of Plumbago rosea L., a rare medicinal plant, via somatic embryogenesis in callus cultures derived from leaf explants was described. Optimum callus formation was achieved on semi-solid Murashige and Skoog (MS) medium supplemented with 0.25 mg dm–3 kinetin and 2.0 mg dm–3 1-naphthaleneacetic acid (NAA). Somatic embryogenesis was achieved upon transferring the 4-week-old callus to a medium containing 1.0 mg dm–3 kinetic (Kn), 0.5 mg dm–3 gibberellic acid (GA3) and 0.1 mg dm–3 NAA. Embryo maturation and germination was achieved on the half-strength MS basal salts supplemented with 0.01 – 0.25 mg dm–3 Kn and 2 % (m/v) saccharose. An average of 50 – 60 plantlets were obtained from 150 mg of embryogenic callus within 4 week of subculture. Out of the 50 plantlets about 28 survived in the greenhouse. |
| |
Keywords: | gibberellic acid naphthaleneacetic acid in vitro medicinal plant |
本文献已被 SpringerLink 等数据库收录! |
|