Mitochondrial malic enzyme in mosaic skeletal muscle of mouse chimeras |
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| Authors: | P. M. Frair P. M. Strasberg K. B. Freeman A. C. Peterson |
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| Affiliation: | (1) Department of Neurosciences, McMaster University, L8S 4J9 Hamilton, Ontario, Canada;(2) Department of Biochemistry, McMaster University, L8S 4J9 Hamilton, Ontario, Canada;(3) Present address: Montreal General Hospital Research Institute, 1650 Cedar Ave, H3G 1A4 Montreal, Quebec, Canada;(4) Present address: Department of Neurosciences, Hospital for Sick Children, M5G 1X8 Toronto, Ontario, Canada |
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| Abstract: | The question was investigated whether mitochondria in the mammalian skeletal muscle fiber syncytium incorporate gene products encoded by one or many nuclei. Mouse chimeras were produced from strains which differ in their electrophoretic variants of the nuclear-coded mitochondrial protein, malic enzyme (MOD-2, E.C. 1.1.1.40, l-malate NADP+ oxidoreductase decarboxylating). The MOD-2 phenotypes of skeletal muscles of these chimeras were characterized in a starch gel electrophoretic system. The results indicate that individual mitochondria can contain products encoded by multiple nuclei and therefore that, for skeletal muscle mitochondria, the cell is not subdivided into nuclear territories. Possible mechanisms of gene product distribution in skeletal muscle fibers are discussed.This work was supported by Grants MT-1940 (K. B. F.) and MA-6411 (A. C. P.) from the Medical Research Council of Canada, and by the Muscular Dystrophy Association of Canada (A. C. P. and P. M. F.). |
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| Keywords: | mitochondrial malic enzyme mitochondrial biogenesis starch gel electrophoresis skeletal muscle mouse chimeras |
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