Culture and serological detection of the xylem-limited bacterium causing citrus variegated chlorosis and its identification as a strain ofXylella fastidiosa |
| |
Authors: | Chung J Chang Dr Monique Garnier Leyla Zreik Victoria Rossetti Joseph M Bové |
| |
Institution: | (1) Laboratory of Cellular and Molecular Biology, INRA and University of Bordeaux II, Villenave d'Ornon Cedex, France;(2) Present address: Department of Plant Pathology, University of Georgia, Georgia Station, 30223 Griffin, GA, USA;(3) Present address: Instituto Biologico, Caixa Postal, 7119-01051 Sao Paulo, SP, Brazil |
| |
Abstract: | A xylem-limited bacterium resemblingXylella fastidiosa has been shown previously by electron mmcroscopy to be associated with citrus variegated chlorosis (CVC), a new disease of sweet organe tress in Brazil. A bacterium was consistently cultured from plant tissues from CVC twigs of sweet orange trees but not from tissues of healthy trees on several cell-free media known to support the growth ofXylella fastidiosa. Bacterial colonies typical ofX. fastidiosa became visible on PW, CS20, and PD2 agar media after 5 and 7–10 days of incubation, respectively. The cells of the CVC bacterium were rod-shaped, 1.4–3 m in length, and 0.2–0.4 m in diameter, with rippled walls. An antiserum against an isolate (8.1.b) of the bacterium gave strong positive reactions to double-antibody-sandwich (DAS), enzyme-linked immunosorbent assay (ELISA) with other cultured isolates from CVC citrus, as well as with several type strains ofX. fastidiosa. This result indicates that the CVC bacterium is a strain ofX. fastidiosa. ELISA was also highly positive with all leaves tested from CVC-affected shoots. Leaves from symptomless tress reacted negatively. Sweet organe seedlings inoculated with a pure culture of the CVC bacterium supported multiplication of the bacterium, which became systemic with 6 months after inoculation and could be reisolated from the inoculated seedlings. Symptoms characteristic of CVC developed 9 months post inoculation. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|