Energy metabolism of autotrophically and heterotrophically grown cells of Nitrobacter winogradskyi |
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Authors: | Hilke Sundermeyer Eberhard Bock |
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Affiliation: | (1) Mikrobiologie, Institut für Allgemeine Botanik der Universität Hamburg, Jungiusstr. 6, D-2000 Hamburg 36, Federal Republic of Germany |
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Abstract: | The adenine nucleotide pools and the NADH pool were compared in intact Nitrobacter winogradskyi cells grown under different conditions. The NADH pool was highest in nitrite-grown cells (22.0 nmol/mg N), less high in acetategrown cells (15.1 nmol/mg N),and lowest in pyruvate-grown cells (11.9 nmol/mg N).The adenine nucleotide pools and the NADH pool were determined after the transition from anaerobic to aerobic conditions.In both autotrophically and heterotrophically grown cells the ATP pool decreased within the first second after the addition of oxygen and then increased.In cells grown with nitrite or acetate the NADH pool increased the first second after the addition of oxygen then decreased below the initial value. In pyruvate-grown cells the changes in the NADH pool were less obvious.In the presence of rotenone autotrophic cells were able to generate ATP, but the reverse energy-dependent electron transport was inhibited. Consequently, NADH was not synthesized. N,N-dicyclohexylcarbodiimide an inhibitor of ATPase, prevented both ATP and NADH generation.Abbreviations DCCD N,N-dicyclohexylcarbodiimide |
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Keywords: | Nitrobacter Intact cells Autotrophic and heterotrophic growth Adenine nucleotide pool NADH pool Oxidative phosphorylation Pyridine nucleotide reduction |
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