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Energy metabolism of autotrophically and heterotrophically grown cells of Nitrobacter winogradskyi
Authors:Hilke Sundermeyer  Eberhard Bock
Institution:(1) Mikrobiologie, Institut für Allgemeine Botanik der Universität Hamburg, Jungiusstr. 6, D-2000 Hamburg 36, Federal Republic of Germany
Abstract:The adenine nucleotide pools and the NADH pool were compared in intact Nitrobacter winogradskyi cells grown under different conditions. The NADH pool was highest in nitrite-grown cells (22.0 nmol/mg N), less high in acetategrown cells (15.1 nmol/mg N),and lowest in pyruvate-grown cells (11.9 nmol/mg N).The adenine nucleotide pools and the NADH pool were determined after the transition from anaerobic to aerobic conditions.In both autotrophically and heterotrophically grown cells the ATP pool decreased within the first second after the addition of oxygen and then increased.In cells grown with nitrite or acetate the NADH pool increased the first second after the addition of oxygen then decreased below the initial value. In pyruvate-grown cells the changes in the NADH pool were less obvious.In the presence of rotenone autotrophic cells were able to generate ATP, but the reverse energy-dependent electron transport was inhibited. Consequently, NADH was not synthesized. N,Nprime-dicyclohexylcarbodiimide an inhibitor of ATPase, prevented both ATP and NADH generation.Abbreviations DCCD N,Nprime-dicyclohexylcarbodiimide
Keywords:Nitrobacter  Intact cells  Autotrophic and heterotrophic growth  Adenine nucleotide pool  NADH pool  Oxidative phosphorylation  Pyridine nucleotide reduction
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