Cell cycle requirements in assembling silent chromatin in Saccharomyces cerevisiae |
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Authors: | Kirchmaier Ann L Rine Jasper |
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Affiliation: | Department of Biochemistry and Purdue Cancer Center, Purdue University, 175 S. University St., West Lafayette, IN 47907, USA. kirchmaier@purdue.edu |
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Abstract: | The establishment of silencing at the silent mating-type locus, HMR, in Saccharomyces cerevisiae requires that yeast pass through S phase of the cell cycle, yet requires neither the initiation of DNA replication at the locus destined to become silenced nor the passage of a replication fork through that locus. We tested whether this S-phase requirement reflects a window within the cell cycle permissive for recruitment of Sir proteins to HMR. The S-phase-restricted event necessary for silencing occurred after recruitment of Sir proteins to HMR. Moreover, cells arrested in early S phase formed silent chromatin at HMR, provided HMR was on a nonreplicating template. Replicating templates required a later step for silencing. These results provide temporal resolution of discrete steps in the formation of silent chromatin and suggest that more than one cell cycle-regulated event may be necessary for the establishment of silencing. |
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