覆盆子提取物联合唑类药物抗真菌活性研究

目的 探讨中药覆盆子提取物联合唑类药物的体外抗真菌作用.方法 采用CLSI公布的M27-A方案微量液基稀释法和棋盘式微量稀释法,测定覆盆子提取物单用及联合唑类药物对不同念珠菌的MIC值和FICI指数.结果 覆盆子不同溶液提取物与氟康唑均表现出协同关系,以覆盆子醇提物为例,单用对念珠菌的MIC80测定值范围主要集中在0.16～1.25 mg/mL,与氟康唑合用后表现出协同关系(FICI≤0.5),且MIC80测定值范围降至0.01 ～0.04 mg/mL;合用后的氟康唑抗真菌活性也明显增强.另外,覆盆子醇提物与不同唑类药物合用后均有协同关系,其MIC80测定值由单用时大于10 mg/mL降至0.04 mg/mL.结论 覆盆子醇提物和唑类药物单用时对耐药念珠菌的抑菌作用较弱,但二者合用后表现出明显的协同关系,对耐药念珠菌的抑菌作用明显增强.

Combination antifungal activity of ethanol extracts from Rubus idaeus and antifungal azoles

Objective To investigate the antifungal activity of extracts from Rubus idaeus combined with azole drugs against Candida spp.in vitro.Methods The minimum inhibitory concentration（MIC）,the fractional inhibitory concentration index（FICI） of extracts from Rubus idaeus and combination with azole drugs against Candida spp.were determined by M27-A Microdilution Method based on CLSI.Results All extracts from Rubus idaeus by different solution methods showed collaboration with fluconazole.For example,the MIC80 of ethanol extracts from Rubus idaeus against Candida spp.mainly concentrated on 0.16~1.25 mg/mL,while combined with fluconazole showed the collaboration（FICI≤0.5）,and the MIC80 reduced to 0.01~0.04 mg/mL.The antifungal effect of fluconazole against Candida spp.was also strengthened.Besides,the MIC80s of ethanol extracts from Rubus idaeus were also reduced from 10 mg/mL to 0.04 mg/mL when being combined with other azole drugs.Conclusion The antifungal effect of ethanol extracts from Rubus idaeus or azole drugs against resistant Candidas was weaker,while the combination showed clear collaboration and the effect against resistant Candida spp.significantly enhanced.