Structural characterization of an O-linked tetrasaccharide from Pseudomonas syringae pv. tabaci flagellin |
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| Authors: | Tomoyuki Konishi Fumiko Taguchi Masako Iwaki Mayumi Ohnishi-Kameyama Masanobu Yamamoto Ikuko Maeda Yoshihiro Nishida Yuki Ichinose Mitsuru Yoshida Tadashi Ishii |
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| Affiliation: | aForestry and Forest Products Research Institute, 1, Matsunosato, Tsukuba, Ibaraki 305-8687, Japan;bGraduate School of National Science and Technology, Okayama University, 1-1-1, Tsushima-naka, Okayama 700-8530, Japan;cNational Food Research Institute, 2-1-12, Kannondai, Tsukuba 305-8642, Japan;dDepartment of Horticulture, Chiba University, 648 Matsudo, Matsudo, Chiba 271-0092, Japan |
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| Abstract: | The flagellin of Pseudomonas syringae pv. tabaci is a glycoprotein that contains O-linked oligosaccharides composed of rhamnosyl and 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methylglucosyl residues. These O-linked glycans are released by hydrazinolysis and then labeled at their reducing ends with 2-aminopyridine (PA). A PA-labeled trisaccharide and a PA-labeled tetrasaccharide are isolated by normal-phase high-performance liquid chromatography. These oligosaccharides are structurally characterized using mass spectrometry and NMR spectroscopy. Our data show that P. syringae pv. tabaci flagellin is glycosylated with a tetrasaccharide, 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methyl-Glcp-(1→3)-α-l-Rhap-(1→2)-α-l-Rhap-(1→2)-α-l-Rha-(1→, as well a trisaccharide, 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methyl-Glcp-(1→3)-α-l-Rhap-(1→2)-α-l-Rha-(1→, which was identified in a previous study. |
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| Keywords: | Bacteria Pseudomonas syringae Flagellin glycosylation O-linked oligosaccharides Structure NMR |
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