The effect of serum on lipid synthesis and on the expression of oligodendrocyte marker-enzymes in oligodendrocyte-enriched glial cells in culture |
| |
Authors: | Jane E C Sykes and Mattxus Lores-Cardozo |
| |
Institution: | Laboratory of Veterinary Biochemistry, State University of Utrecht, P.O. Box 80177, 3508 TD, Utrecht, The Netherlands |
| |
Abstract: | Glial cells were isolated from the cerebra of 7-day old rats and the effect of serum on the development of these cells in culture was studied. The activities of the oligodendrocyte marker-enzymes, 2′3′-cyclic nucleotide 3′-phosphodiesterase and glycerol 3-phosphate dehydrogenase and the synthesis of the myelin-associated sulpholipid, sulphatide, were used to monitor the differentiation of these cells in vitro. The results indicate that serum: (i) represses lipogenesis, cholesterogenesis and sulphatide synthesis, (ii) lowers the expression of 2′3′-cyclic nucleotide 3′ phosphodiesterase and glycerol 3-phosphate dehydrogenase but not of lactate dehydrogenase and (iii) thus impairs the differentiation of oligodendrocytes. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|