QUANTIFICATION OF ENTRY INTO AND EXIT FROM THE CELL CYCLE IN HUMAN LYMPHOCYTE CULTURES |
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| Authors: | R. Dorian J. L. Bernheim J. Mendelsohn |
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| Affiliation: | Division of Hematology and Oncology, Department of Medicine, University of California, San Diego, La Jolla, California, U.S.A. |
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| Abstract: | A mathematical model is presented for the analysis of transition between cycling and non-cycling compartments by cells responding to a growth stimulus. the cellular age distribution as a function of time is derived from sequential [3H]thymidine pulse labeling indices. Rates of entry into and exit from the cycling compartment are determined on the basis of labeling indices obtained after instantaneous and long duration [3H]thymidine pulses. Analysis of an experiment involving sequential measurements over the whole lifespan of a human lymphocyte culture stimulated by phytohemagglutinin is presented as an example of the application of this method. |
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