Taxon-specific multiplex-PCR for quick,easy, and accurate identification of encyrtid and aphelinid parasitoid species attacking soft scale insects in California citrus groves |
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Authors: | Paul F Rugman-Jones Lisa D Forster Emilio Guerrieri Robert F Luck Joseph G Morse Maurilia M Monti Richard Stouthamer |
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Institution: | 1.Department of Entomology,University of California,Riverside,USA;2.Istituto per la Protezione delle Piante, Consiglio Nazionale delle Ricerche,Portici,Italy |
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Abstract: | Citricola scale, Coccus pseudomagnoliarum Kuwana (Hemiptera: Coccidae), is a serious pest of citrus in California’s San Joaquin Valley, but not in southern California
where a complex of Metaphycus spp. Mercet (Hymenoptera: Encyrtidae) suppress it. This has created interest in using these (and other Metaphycus) species for biological control in the San Joaquin Valley. A critical step in assessing an organism’s potential for biological
control is the ability to accurately identify it. For Metaphycus spp., this currently requires slide mounted adult specimens and expert taxonomic knowledge. We present a simple, quick and
accurate method to identify any life stage of the ten major parasitoids of soft scales in California citrus, based on amplification
of ribosomal DNA, using the polymerase chain reaction (PCR). Three multiplex-PCR protocols amplify products of taxon-specific
sizes, allowing direct diagnosis of taxa accommodated by the PCR, and reducing identification time to a fraction of that of
existing methods. |
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