Isolation and characterization of halophilic bacteria from Urmia Lake in Iran |
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Authors: | Sepideh Zununi Vahed Haleh Forouhandeh Salar Hassanzadeh Hans-Peter Klenk Mohammad Amin Hejazi Mohammad Saeid Hejazi |
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Affiliation: | (1) Rice Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan, China, 611130;(2) Key Laboratory of Southwest Crop Gene Resource & Genetic Improvement of Ministry of Education, Sichuan Agricultural University, Ya’an, Sichuan, China, 625014;(3) Agriculture and Agri-Food Canada, 960 Carling Ave, Ottawa, Ontario, Canada, K1A 0C6;(4) Rice Research Institute, Dongbei Road No. 555, Liucheng Town, Wenjiang, 611130, Sichuan, People’s Republic of China; |
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Abstract: | Urmia Lake is one of the most permanent hypersaline lakes in the world which is threatened by hypersalinity and serious dryness. In spite of its importance no paper has been published regarding bacterial community of this lake. Accordingly, the present study aimed to investigate the halophilic bacteria in the aforementioned lake. In so doing, thirty seven strains were isolated on six different culture media. The isolated strains were characterized using phenotypic and genotypic methods. Growth of the strains occurred at 25–35°C, pH 6–9 and 7 to 20% (w/v) NaCl indicating that most of the isolates were moderately halophiles. Catalase, oxidase and urease activities were found to be positive for the majority of the isolates. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolated bacteria belonged to two major taxa: Gammaproteobacteria (92%, including Salicola [46%], Pseudomonas [13.5%], Marinobacter [11%], Idiomarina [11%], and Halomonas [8%]) and Firmicutes (8%, including Bacillus [5%] and Halobacillus [3%]). In addition, a novel bacterium whose 16S rRNA gene sequence showed almost 98% sequence identity with the taxonomically troubled DSM 3050T, Halovibrio denitrificans HGD 3T and Halospina denitriflcans HGD 1–3, each, was isolated. 16S rRNA gene similarity levels along with phenotypic characteristics suggest that some of the isolated strains could be regarded as potential type strain for novel species, on which further studies are recommended. |
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