The <Emphasis Type="Italic">paleoAP3</Emphasis>-type gene <Emphasis Type="Italic">CpAP3</Emphasis>, an ancestral B-class gene from the basal angiosperm <Emphasis Type="Italic">Chimonanthus praecox</Emphasis>, can affect stamen and petal development in higher eudicots |
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Authors: | Qiong Zhang Bei-Guo Wang Ke Duan Li-Gang Wang Meng Wang Xue-Ming Tang Ai-Hu Pan Shun-Zhao Sui Guang-Dong Wang |
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Institution: | (1) Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Biotechnology, Shanghai Academy of Agricultural Sciences (SAAS), Beidi Road 2901, Minhang District, Shanghai, 201106, China;(2) College of Horticultural Sciences, Nanjing Agricultural University, Nanjing, 210095, China;(3) College of Horticulture and Landscape, Institute of Ornamental Research, Southwest University, Chongqing, 400716, China; |
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Abstract: | Wintersweet (Chimonanthus praecox), a basal angiosperm endemic to China, has high ornamental value for developing beautiful flowers with strong fragrance.
The molecular mechanism regulating flower development in wintersweet remains largely elusive. In this project, we seek to
determine the molecular features and expression patterns of the C. praecox paleoAP3-type gene CpAP3 and examine its potential role in regulating floral development via ectopic expression in Arabidopsis thaliana and Petunia hybrida. The expression of CpAP3 is tissue-specific, with the highest level in the tepals, moderate level in carpels, and weak levels in stamen and vegetative
stem tissues. Its dynamic expression during flowering is associated with flower-bud formation. Ectopic expression of CpAP3 partially rescued stamen development in ap3 mutant Arabidopsis. Although no phenotypic effect has been observed in wild-type Arabidopsis, CpAP3 overexpression in petunia brought rich morphological changes and homeotic conversions to flowers, mainly involving disruption
of petal and stamen development. Expressed in a broader range than those canonical B-function regulators, the ancestral B-class
gene CpAP3 can affect petal and stamen development in higher eudicots. This gene also holds some bioengineering potential in creating
novel floral germplasms. |
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