Long-range physical maps of two loci (Aps-1 and GP79) flanking the root-knot nematode resistance gene (Mi) near the centromere of tomato chromosome 6 |
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Authors: | Raymond A. J. J. van Daelen Frans Gerbens Fred van Ruissen Jac Aarts Jan Hontelez Pim Zabel |
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Affiliation: | (1) Department of Molecular Biology, Wageningen Agricultural University, Dreyenlaan 3, 6703 HA Wageningen, Netherlands;(2) Present address: Keygene NV, Agrobusiness Park 93, 6703 PW Wageningen, Netherlands |
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Abstract: | The root knot nematode resistance gene Mi in tomato has been mapped in the pericentromeric region of chromosome 6. With the objective of isolating Mi through a map-based cloning approach, we have previously identified and ordered into a high-resolution genetic linkage map a variety of tightly linked molecular markers. Using pulsed-field gelelectrophoresis and various rarely cutting restriction enzymes in single, double and partial digestions, we now report long-range physical maps of the two closest flanking markers, acid phosphatase-1 (Aps-1) and GP79, which span over 400 and 800 kb, respectively. It is concluded that the physical distance between both markers is larger than predicted on the basis of genetic linkage analysis. Furthermore, two RFLP markers (H3F8 and H4H10) which map genetically to the same locus as Aps-1 do not show physical linkage, indicating severe suppression of recombination in this region of the chromosome. Finally, no evidence was obtained showing the presence of a CpG island near Aps-1. |
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Keywords: | acid phosphatase gene centromere root knot nematode resistance gene pulsed-field gel electrophoresis tomato |
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