Nucleotide sequence of a highly repeated DNA sequence and its chromosomal localization in Allium fistulosum |
| |
| Authors: | K. Irifune K. Hirai J. Zheng R. Tanaka H. Morikawa |
| |
| Affiliation: | (1) Graduate Department of Gene Science, Faculty of Science, Hiroshima University, 724 Higashi-Hiroshima, Japan;(2) Present address: Shobara Kakuchi High School, Shobara, 727 Hiroshima, Japan;(3) Present address: Department Biology, Nankai University, 300071 Tianjin, China |
| |
| Abstract: | A highly repeated DNA sequence with a repeating unit of approximately 380bp was found in EcoRV digests of the total genomic DNA of Allium fistulosum. Three independent clones containing this unit were isolated, and their repeating units sequenced. These units showed more than 94% sequence homology, and the copy number was estimated to be about 2.8×106 per haploid genome. In situ hybridization, with the repeating unit as a probe, and C-banding analyses indicated that the repeated DNA sequence of A. fistulosum is closely associated with the major C-heterochromatin in the terminal regions of all 16 chromosomes at mitotic metaphase. The characters of the repeating unit are similar to those of the A. cepa unit, which is taxonomically closely related to A. fistulosum. |
| |
| Keywords: | Allium fistulosum Repeated DNA sequence In situ hybridization Nucleotide sequence C-banding |
| 本文献已被 SpringerLink 等数据库收录！ |
|
