Bovine serum albumin-hemoglobin fractionation: significance of ultrafiltration system and feed solution characteristics |
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| Authors: | Rishi Shukla Malini Balakrishnan Gopal P Agarwal |
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| Institution: | (1) Agricultural Bioprocess Laboratory, University of Illinois at Urbana-Champaign, 1302 West Pennsylvania Avenue, Urbana, IL 61801, USA;(2) Darbari Seth Block, India Habitat Center, Tata Energy Research Institute, Lodi Road, New Delhi, 110003, India;(3) Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi, 110016, India |
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| Abstract: | This work investigates the fractionation of similar molecular weight proteins bovine serum albumin (69 kD) and bovine hemoglobin (67 kD) by ultrafiltration. Three different membranes, viz. regenerated cellulose, poly(sulfone) and surface modified poly(acrylonitrile), each with a nominal molecular cutoff rating of 100 kD, were examined. The experiments were conducted in dead end, crossflow and vortex flow filtration modes and the separation was studied as a function of feed pH and ionic strength. Under similar system hydrodynamics, the surface modified poly(acrylonitrile) membrane displayed the highest resolution with minimum membrane fouling. The separation could be improved further by operating at low applied pressure (40 kPa) and high mass transfer (> 20 × 10–6 m/s) in a vortex flow module. Under these conditions, the highest separation factor of 40 was obtained at the pI of hemoglobin. |
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| Keywords: | bovine serum albumin hemoglobin protein fractionation separation factor ultrafiltration vortex flow filter |
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