Studies on the disaggregation of EF-1 with carboxypeptidase A.

The ability of partially purified phospholipase C preparations (Bacillus cereus) to disaggregate elongation factor 1 from rabbit reticulocytes is due to the presence of carboxypeptidase A in the preparations. A similar factor has been purified from the growth medium of B. cereus. In contrast, the disaggregation of elongation factor 1 observed with extracts of Artemia salina nauplii appears to be due to a protease. These results show that different types of proteolytic modification of elongation factor 1 can result in disaggregation of the factor.