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Liposome-protoplast fusion in Phycomyces blakesleeanus
Authors:José Arnau  Antonio Ortiz  Juan C Gomez-Fernández  Francisco J Murillo  Santiago Torres-Martínez
Institution:Departamento de Genética y Microbiología, Facultad de Biología, Universidad de Murcia, Murcia, Spain;Departamento de Bioquímica, Facultad de Veterinaria, Universidad de Murcia, Murcia, Spain
Abstract:Abstract We describe here fusion between phospholipid vesicles (liposomes) and protoplasts to the fungus Phycomyces blakesleeanus . Both 6-carboxyfluorescein and the kanamycin resistance harboured by the plasmid have been transferred from liposomes to protoplasts of Phycomyces by the fusion technique.
Keywords:Plasmid pPS11  Transformation  6-Carboxyfluorescein
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