Analysis of gene expression profile in pollen development of recessive genic male sterile <Emphasis Type="Italic">Brassica napus</Emphasis> L. line S45A |
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Authors: | Yuning Chen Shaolin Lei Zhengfu Zhou Fangqin Zeng Bin Yi Jing Wen Jinxiong Shen Chaozhi Ma Jinxing Tu Tingdong Fu |
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Institution: | (1) National Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, 430070 Wuhan, People’s Republic of China |
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Abstract: | Male sterility in a near-isogenic line S45AB after 25 generations of subcrossing is controlled by two pairs of duplicate genes.
The genotype of S45A is Bnms1Bnms1Bnms2Bnms2, and that of S45B is BnMs1Bnms1Bnms2Bnms2, respectively. Histological observations revealed that abnormal anther development appeared in the tapetum and pollen exine
during the tetrad stage. This male sterility was characterized by hypertrophy of the tapetal cells at the tetrad stage and
a complete lack of microspore exine after the release of microspores from the tetrads. To elucidate the mechanism of this
recessive genic male sterility, the flower bud expression profiles of the S45A and S45B lines were analyzed using an Arabidopsis thaliana ATH1 oligonucleotide array. When compared with the S45B line, 69 genes were significantly downregulated, and 46 genes were
significantly upregulated in the S45A line. Real-time polymerase chain reaction (PCR) was then used to verify the results
of the microarray analysis, and the majority of the downregulated genes in the S45A line were abundantly and specifically
expressed in the anther. The results of the real-time PCR suggest that Bnms1 might be involved in the metabolism of lipid/fatty acids, and the homologous mutation of Bnms1 may either block the biosynthesis of sporopollenin or block sporopollenin from being deposited on the microspore surface,
thus, preventing pollen exine formation. The role of Bnms1 in the regulatory network of exine formation is also discussed as well.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Brassica napus Pollen exine Tapetum Real-time PCR Microarray Male sterility |
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