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Analysis of ribosomal DNA restriction patterns in the genusKluyveromyces
Authors:Peng Shen  Shung-Chang Jong  Francis I Molina
Institution:(1) Mycology and Botany Department, American Type Culture Collection, 12301 Parklawn Drive, 20852 Rockville, MD, USA
Abstract:Riboprinting was used to determine the relationships among strains belonging to 15 species of the genusKluyveromyces. The small subunit ribosomal RNA gene (SSU rDNA) was amplified using the Polymerase Chain Reaction (PCR) and subjected to a battery of nine restriction enzymes. Similarity coefficients between strains were calculated based on shared and unique restriction fragments. Cluster analysis revealed three major groups that generally correlated with previously reported relationships based on other molecular data. Variations in SSU rDNA restriction fragments may be used for differentiation of theKluyveromyces strains included in this study.The U.S. Government right to retain a non-exclusive, royalty free licence in and to any copyright is acknowledged.
Keywords:Kluyveromyces  Polymerase Chain Reaction (PCR)  riboprinting  Small Subunit ribosomal RNA gene (SSU rDNA)
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