Standardization of reagents and methods used in cytological and histological practice with emphasis on dyes, stains and chromogenic reagents |
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| Authors: | H. O. Lyon A. P. De Leenheer R. W. Horobin W. E. Lambert E. K. W. Schulte B. Van Liedekerke D. H. Wittekind |
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| Affiliation: | (1) Department of Pathology 134, Hvidovre Hospital, University of Copenhagen, DK-2650 Hvidovre, Denmark;(2) Laboratoria voor Medische Biochemie en voor Klinische Analyse, Universiteit Gent, B-9000 Gent, Belgium;(3) Department of Biomedical Science, University of Sheffield, S10 2TN Sheffield, UK;(4) Anatomisches Institut, Johannes Gutenberg-Universität Mainz, D-55122 Mainz, Germany;(5) Anatomisches Institut II, Universität Freiburg, D-79001 Freiburg im Breisgau, Germany |
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| Abstract: | Summary The need for the standardization of reagents and methods used in the histology laboratory is demonstrated. After definitions of dyes, stains, and chromogenic reagents, existing standards and standards organizations are discussed. This is followed by practical instructions on how to standardize dyes and stains through the preparation of reference materials and the development of chromatographic methods. An overview is presented of the problems concerned with standardization of the Romanowsky-Giemsa stain for cytological and histological application. Finally, the problem of how to convince routine dye and stain users of the need for standardization in their histology laboratories is discussed. |
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