Leptin improves in-vitro maturation of goat oocytes through MAPK and JAK2/STAT3 pathways and affects gene expression of cumulus cells |
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| Affiliation: | 1. Laboratório de Biotécnicas Aplicadas à Reprodução, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil;2. Department of Dairy Science, University of Wisconsin-Madison, Madison, WI, 53706 USA;3. Instituto Agronômico de Pernambuco, Arcoverde, Pernambuco, Brazil;1. Department of Gynecologic Oncology, Shaanxi Provincial Cancer Hospital, Xi’an 710061, China;2. Department of Gastrointestinal Oncology, Shaanxi Provincial Cancer Hospital, Xi’an 710061, China;1. Department of Gynecology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, China;2. Department of Dermatology, Shanghai Baoshan Hospital of Integrated Traditional Chinese and Western Medicine, China;3. Department of Medical Imaging, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, China;1. Embryo Biotechnology Laboratory (EmBio Lab), Department of Animal Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran;2. Department of Physiology, McGill University, Montreal, QC, Canada;3. Department of Biology, McGill University, Montreal, QC, Canada;4. Department of Bioengineering, McGill University, Montreal, QC, Canada;1. Department of Gynecology, Henan Provincial People’s Hospital; People’s Hospital of Zhengzhou University; People’s Hospital of Henan University, Zhengzhou, 450003, Henan, China;2. Department of Gynecology, Zhou kou Central Hospital, Zhoukou 466000, Henan, China;3. Department of Obstetrics and Gynecology, Zhoukou Maternal and Child Health Care, Zhoukou, 466000, Henan, China;1. Department of Laboratory, The Affiliated Changzhou Second People’s Hospital of Nanjing Medical University, Changzhou, Jiangsu, China;2. Department of Gynecology, The Affiliated Changzhou Second People’s Hospital of Nanjing Medical University, Changzhou, Jiangsu, China |
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| Abstract: | We investigated whether the recombinant leptin (1, 10, 100 ng/mL) influences the meiotic maturation of goat oocytes, whether the MAPK and JAK2/STAT3 pathways mediate the effects of leptin during in-vitro maturation, and whether leptin differently affects the abundance of mRNAs relevant to leptin signal transduction and apoptosis in oocytes and cumulus cells. The addition of leptin to the maturation medium positively affected the number of oocytes that completed nuclear maturation. Nuclear oocyte maturation stimulated by leptin was significantly impaired when we added the specific inhibitors of MAPK (U0126) and JAK2/STAT3 (AG490) to the maturation medium. The addition of leptin (10 ng/mL) during maturation did not affect the expression of AMPKα1, PPARα, Caspase 3, and BCL2 genes in oocytes or cumulus cells. The PPARγ and BAX mRNA abundances were significantly reduced in cumulus cells in the leptin group compared to the control group. Our results demonstrate that supplementation of the in-vitro maturation medium with leptin significantly improves nuclear maturation and reveal the important role of the MAPK and JAK2/STAT3 signaling pathways in establishing the leptin-mediated nuclear maturation of goat oocytes. Moreover, leptin treatment affects PPARγ and BAX gene expression in cumulus cells. |
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| Keywords: | Caprine Meiosis PPARγ BAX qRT-PCR |
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