OsASHL1 and OsASHL2, two members of the COMPASS-like complex,control floral transition and plant development in rice |
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| Affiliation: | 1. Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, Heilongjiang 150081, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang 150030, China;4. College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China;1. The Sichuan Provincial Key Laboratory for Human Disease Gene Study and Department of Laboratory Medicine, Center for Medical Genetics, Sichuan Provincial People''s Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072, China;2. Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences (2019RU026), Sichuan Academy of Medical Sciences and Sichuan Provincial People''s Hospital, Chengdu, Sichuan 610072, China;3. Key Laboratory of Tibetan Medicine Research, Chinese Academy of Sciences and Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Xining, Qinghai 810008, China;1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070, China;2. Hubei Hongshan Laboratory, Wuhan, Hubei 430070, China;1. Departments of Neonatology, Children''s Hospital of Fudan University, National Children''s Medical Center, Shanghai 201102, China;2. Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China;3. Key Laboratory of Birth Defects, Children''s Hospital of Fudan University, National Children''s Medical Center, Shanghai 201102, China;4. Department of Biostatistics, School of Public Health, Fudan University, Shanghai 200032, China;5. Department of Obstetrics, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200090, China;6. Stem Cell Center, Children''s Hospital of Fudan University, Shanghai 201102, China;7. MOE Frontiers Center for Brain Science, Fudan University, Shanghai 201102, China;1. The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, School of Life Sciences, Ministry of Education Key Laboratory for Membraneless Organelles & Cellular Dynamics, The USTC RNA Institute, Department of Obstetrics and Gynecology, Biomedical Sciences and Health Laboratory of Anhui Province, University of Science and Technology of China, Hefei, Anhui 230001, China;2. CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Hefei, Anhui 230027, China |
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| Abstract: | COMPASS or COMPASS-like is a highly conserved polyprotein complex in eukaryotes that is often involved in methylation of histone H3 lysine 4 (H3K4). However, the biological function of this complex in rice (Oryza sativa) is unclear. Here, we report the identifiction of their functions in growth and development. The osashl1 osashl2 double mutant shows a dwarf and late-flowering phenotype. Lower expression of Ehd1, OsVIL4, and OsMADS51 in the osashl1 osashl2 double mutant background accompanies a delayed vegetative growth phase and photoperiod-sensitive phase compared with that in wild type. Notably, there is less H3K4 mono-, di- and tri-methylation genome-wide in the double mutant, in particular less H3K4 tri-methylation at OsVIL4. Consistent with this result, knockout of OsVIL4 gives rise to a late-flowering phenotype similar to that of the osashl1 osashl2 double mutant, suggesting that OsVIL4 is a target of the COMPASS-like complex. In addition, the expression of key genes in brassinosteroid and gibberellic acid metabolism is altered in the osashl1 osashl2 double mutant, suggesting that the COMPASS-like complex regulates plant growth and development by modulating the levels of these two phytohormones. In summary, we demonstrate that OsASHL1 and OsASHL2 are important for floral transition and plant development. |
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| Keywords: | Flowering COMPASS-like H3K4 methylation |
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