microRNAs in the blastocoel fluid as accessible indicators of chromosomal normality |
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| Institution: | 1. Immunology Research Center, Tabriz University of Medical Science, Tabriz, Iran;2. Department of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran;3. Women''s Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;4. Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran;1. Health Management Center, Huizhou Central People''s Hospital, Huizhou, Guangdong, China;2. Department of Stomatology, Huizhou Central People''s Hospital, Huizhou, Guangdong, China;3. Department of Breast Surgery, Huizhou Central People''s Hospital, Huizhou, Guangdong, China;1. Pelvic Floor and Postpartum Rehabilitation Center, Changsha Maternal and Child Health Care Hospital, Changsha, Hunan 410007, PR China;2. Department of Gynaecology, Changsha Maternal and Child Health Care Hospital, Changsha, Hunan 410007, PR China;1. Werlhof-Institute Hannover, Schillerstr. 23, 30159 Hannover, Germany;2. Institute for Anatomy, University of Veterinary Medicine Hannover Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany;3. Friedrich-Loeffler-Institute (FLI), Institute of Farm Animal Genetics (ING), Höltystrasse 10, 31535 Neustadt, Germany;1. Department of Gynecology, Shenzhen People’s Hospital, Shenzhen 518100, Guangdong, PR China;2. Department of Clinical Laboratory, Yantian People’s Hospital of Southern University of Science and Technology, Shenzhen 518083, Guangdong, PR China;1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Animal Reproduction Institute, College of Animal Science and Technology, Guangxi University, Nanning 530004, China;2. Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;3. Key Laboratory of Animal Breeding and Reproduction, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;4. Suqian Institute of Agricultural Sciences, Jiangsu Academy of Agricultural Sciences, Suqian 223800, China;5. Reproductive Medical Center, Renmin Hospital of Wuhan University, JieFang Road 238, Wuhan 430060, China;1. Department of Gynecology and Obstetrics, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China;2. Reproductive Medicine Center, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China |
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| Abstract: | MicroRNAs (miRNAs) derived from the pre-implantation blastocoel fluid (BF) have attracted interest as accessible biomarkers indicative of embryonic health in ongoing IVF cycles. Therefore, we investigated expression levels of some aneuploidy-associated miRNAs and implantation-related mRNAs as predictive markers for embryo chromosomal normality. In this study, the BF of 25 blastocysts that had been checked for aneuploidy (aneuploid=17 and euploid=8) was aspirated and the expression of 10 miRNAs (miR-20a, miR-30c, miR‐661, miR-372, miR-142, miR-191, miR‐345, miR‐339, miR‐141, and miR-27b) and four genes (ERBB4, SELL, ITGB3, ITGAV) were evaluated using real time-PCR. Results showed that the levels of miR-661 and miR-20a were significantly higher in the BF of the aneuploid embryos compared to the euploid group (p = 0.0017 and 0.004, respectively). A comparison of the mRNA levels between the aneuploid and euploid groups also demonstrated a significant difference in ITGAV (p = 0.013) and SELL (p = 0.0317) levels. In the euploid group, a negative correlation was found between ITGB3 and miR-30c (r = ?0.71, p = 0.08), and in the aneuploid group, a positive correlation was found between ERBB4 and miR-345 (r = 0.71, p = 0.02). It can be suggested that miR-20a, miR-661, and ITGAV levels of BF could be used as less-invasive biomarkers to evaluate embryonic health. Moreover, aneuploidy-related miRNA levels were associated with levels of genes involved in embryo implantation. |
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| Keywords: | Blastocoel fluid Biomarker Implantation In vitro fertilization MiRNAs |
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