| 短小芽孢杆菌2080碱性蛋白酶的纯化与性质 |
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| 引用本文： | 李祖明,李鸿玉,王德良,莫欣,白志辉,李丽云. 短小芽孢杆菌2080碱性蛋白酶的纯化与性质[J]. 工业微生物, 2009, 39(3): 6-10. DOI: 10.3969/j.issn.1001-6678.2009.03.002 |
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| 作者姓名： | 李祖明 李鸿玉 王德良 莫欣 白志辉 李丽云 |
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| 作者单位： | 北京联合大学师范学院,北京,100011;中国食品发酵工业研究院,北京,100027;中科院生态环境研究中心,北京,100085 |
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| 基金项目： | 北京市教委科研计划项目，北京市属市管高校人才强教计划，北京市优秀人才培养资助项目 |
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| 摘 要： | 短小芽孢杆菌（Bacillus pumilus）2080碱性蛋白酶的发酵液经超滤、硫酸铵沉淀、CM Sepharose Fast Flow和DEAE Sepharose Fast Flow离子交换层析得到了纯化的组分。SDS-PAGE电泳分析显示其分子量约为61kDa。酶学性质研究表明，该纯化酶的最适pH为10．5，最适温度为50℃。
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| 关 键 词： | 碱性蛋白酶 短小芽孢杆菌 纯化 性质 |
Purification and properties of alkaline protease from Bacillus pumilus 2080 |
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| LI Zu-ming,LI Hong-yu,WANG De-liang,MO Xin,BAI Zhi-hui,LI Li-yun. Purification and properties of alkaline protease from Bacillus pumilus 2080[J]. Industrial Microbiology, 2009, 39(3): 6-10. DOI: 10.3969/j.issn.1001-6678.2009.03.002 |
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| Authors: | LI Zu-ming LI Hong-yu WANG De-liang MO Xin BAI Zhi-hui LI Li-yun |
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| Affiliation: | LI Zu-ming, LI Hong-yu , WANG De-liang, MO Kin , BAI Zhi-hui, LI Li-yun (1. Teacher＇s College of Beijing Union University, Beijing 100011; 2. China National Research Institute of Food ＆ Fermentation Industries; 3. The Research Center for Eco-Envircrnental Sciences, Chinese Academy of Sciences, Beijing 100085) |
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| Abstract: | A high yielding alkaline protease was obtained from Bacillus pumilus 2080 by solid-state fermentation. The alkaline protease from the crude enzyme were purified by ultra-filtration, ammonium sulphate precipitation, CM sepharose fast flow chromatography and DEAE sepharose fast flow chromatography. The purified alkaline protease was demonstrated to be electrophoretic homogeneity by SDS-PAGE, with a molecular weight of about 61 kDa. The optimal pH and temperature for activity of the purified alkaline protease was 10.5 and 50 12 respectively. |
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| Keywords: | alkaline protease Bacillus lmmilus purification properties |
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