Trichoderma reesei cellobiohydrolase II is associated with the outer membrane when overexpressed in Escherichia coli |
| |
Authors: | Diya M Abdeljabbar Hank J Song A James Link |
| |
Institution: | (1) Department of Chemical & Biological Engineering, Princeton University, Princeton, NJ 08544, USA;(2) Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA;(3) A207 Engineering Quadrangle, Princeton University, Princeton, NJ 08544, USA; |
| |
Abstract: | Cellulose degradation is essential for the future production of many advanced biofuels. Cellulases from the filamentous fungus
Trichoderma reesei are among the most efficient enzymes for the hydrolysis of cellulosic materials. One of the cellulases from T. reesei, cellobiohydrolase II (CBH2), was studied because of its industrial relevance and proven enzymatic activity. Using both crude
and rigorous membrane fractionation methods we show that full length T. reesei CBH2 is exclusively localized to the outer membrane when expressed recombinantly in Escherichia coli. Even fusing signal sequence-free maltose-binding protein to the N-terminus of CBH2, which has been shown to increase solubility of other proteins, did not prevent the outer membrane localization
of CBH2. These results highlight the difficulties in producing fungal cellulases in bacterial hosts and provide a stepping
stone for future cellulase engineering efforts. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|