Cloning, sequencing, and expression of the Escherichia coli peptide methionine sulfoxide reductase gene. |
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Authors: | M A Rahman H Nelson H Weissbach N Brot |
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Institution: | Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110. |
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Abstract: | The gene encoding peptide methionine sulfoxide reductase was cloned from an Escherichia coli genomic library using an oligonucleotide probe based on the amino-terminal sequence of the protein. The nucleotide sequence revealed that the gene codes for a polypeptide of 212 amino acid residues with a calculated molecular weight of 23,314. The protein has been overexpressed in E. coli and is present as a soluble active species. |
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