Identification of the domains required for the localization of Prp19p to lipid droplets or the nucleus |
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Authors: | Cho Si Young Park Pil Joon Lee Jeong Ho Kim Jung Joo Lee Tae Ryong |
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Institution: | aR & D Center, AmorePacific Corporation, 314-1 Bora-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-729, Republic of Korea |
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Abstract: | Prp19p is a protein found in the nucleus, cytosol or lipid droplets depending on the cell type. Prp19p participates in pre-mRNA splicing, in neuronal/astroglial cell fate decisions or in adipocyte lipid droplet biogenesis. In this study, the motifs of Prp19p that are necessary for its localization to lipid droplets or the nucleus in 3T3-L1 adipocytes are investigated using a series of truncated mutants of Prp19p that were fused to EGFP and transiently introduced into differentiated 3T3-L1 adipocytes. Immunofluorescence microscopy revealed that a domain of amino acids 167–250 is necessary for the recruitment of Prp19p to lipid droplets and that a domain of amino acids 1–166 is necessary for the recruitment of Prp19p to a nucleus. |
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Keywords: | Prp19p Adipocytes Lipid droplet Nucleus Localization signal |
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