Diversity of Arsenate Reductase Genes (<Emphasis Type="Italic">arsC</Emphasis> Genes) from Arsenic-Resistant Environmental Isolates of <Emphasis Type="Italic">E. coli</Emphasis> |
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Authors: | Sukhvinder Kaur Majid Rasool Kamli Arif Ali |
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Institution: | (1) Gene Expression Laboratory, Department of Biosciences, Jamia Millia Islamia, New Delhi, 110025, India;(2) Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110025, India |
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Abstract: | A polymerase chain reaction (PCR) approach was used to assess the occurrence and diversity of arsenate reductase gene (arsC gene) in arsenic-resistant environmental E. coli strains. For this purpose, two different sets of primers were designed for the specific amplification of approximately 370-bp
fragments from the arsC gene. These primers were used to screen a collection of 25 environmental arsenic-resistant strains isolated from different
geographical regions of India, as well as Bangladesh. The PCR results showed that 17 out of the 25 environmental isolates
(68%) contained a gene related to the arsC family. Phylogenetic analysis of the protein sequences deduced from the amplicons indicated a prevalence of arsC genes in the isolated strains. A significant divergence in the DNA sequence was found in the arsC genes among As-resistant environmental E. coli strains from this study, and arsenic resistance, a genetic character, arose from a common ancestral background. |
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