| 大肠杆菌K88体外黏附Caco-2细胞及其对细胞膜的影响 |
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| 引用本文： | 郭彤,许梓荣.大肠杆菌K88体外黏附Caco-2细胞及其对细胞膜的影响[J].细胞生物学杂志,2004,26(2):177-180. |
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| 作者姓名： | 郭彤 许梓荣 |
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| 作者单位： | 郭彤（浙江大学动物分子营养学教育部重点实验室,浙江大学饲料科学研究所,杭州,310029）
许梓荣（浙江大学动物分子营养学教育部重点实验室,浙江大学饲料科学研究所,杭州,310029） |
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| 摘 要： | 采用体外Caco-2细胞培养模型，研究大肠杆菌K88黏附Caco-2肠上皮细胞后对其存活率及增殖活力、细胞膜磷脂酶A2、细胞内Ca^2 浓度及膜流动性的影响。结果表明，细菌黏附3h后细胞活力明显下降，PLA2活性升高，细胞内Ca^2 浓度增加，细胞膜流动性降低，从而导致肠上皮细胞膜结构和功能的损害。
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| 关 键 词： | 大肠杆菌K88 Caco-2细胞 细胞膜 体外黏附 存活率 |
| 文章编号： | 0253-9977(2004)02 |
| 修稿时间： | 2003年7月12日 |
Effects of Escherichia coli K88 Adhesion on Membrane Characteristics of Caco-2 Cells in vitro |
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| GUO Tong,XU Zi Rong.Effects of Escherichia coli K88 Adhesion on Membrane Characteristics of Caco-2 Cells in vitro[J].Chinese Journal of Cell Biology,2004,26(2):177-180. |
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| Authors: | GUO Tong XU Zi Rong |
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| Abstract: | Caco-2 intestinal epithelial cells were used in this experiment and adhered with Escherichia coli K88. The change in the viability of the cells and the activity of membranous PLA2, the calcium concentration of cells and membrane fluidity were studied. Results showed: at 3h after the adhesion of Escherichia coli K88, the viability of the Caco-2 cells decreased significantly; the activity of membranous PLA2 and the calcium concentration of cells increased significantly; cell membrane fluidity decreased. Therefore, structure and function of Caco-2 cell membrane suffered impairment. |
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| Keywords: | Escherichia coli K88 adhesion Caco-2 cell cell membrane |
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