Heterokaryon Identification Through Simultaneous Fluorescence of Tetramethylrhodamine Isothiocyanate and Fluorescein Isothiocyanate Labelled Protoplasts |
| |
Authors: | Tina L. Barsby Stephen A. Yarrow James F. Shepard |
| |
Affiliation: | a Department of Plant Pathology, Kansas State University, Manhattan, Kansas |
| |
Abstract: | Protoplasts were separately stained with the fluorescent dyes fluorescein iso-thiocyanate (FITC) and tetramethylrhodamine isothiocyanate (TRITC). Following fusion, doubly stained heterokaryons were identified under fluorescence microscopy by using the Zeiss filter set 48 77 05 (excitation filter 450-490 nm, dichroic reflector 510 nm, and barrier filter 520 nm) which allowed simultaneous fluorochrome emissions. Previously, either emisson spectrum, but not both, was possible for any single filter set. |
| |
Keywords: | |
本文献已被 InformaWorld 等数据库收录! |
|