Azide-resistant mutants in Acinetobacter calcoaceticus A2 are defective in protein secretion |
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Authors: | Adi Elkeles Eugene Rosenberg Eliora Z Ron |
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Institution: | Department of Molecular Microbiology and Biotechnology, The George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel |
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Abstract: | Abstract Azide, an inhibitor of ATPase, and a specific inhibitor of protein export was used in order to select for protein secretion mutants in Acinetobacter calcoaceticus A2. Two such mutants were isolated that were azide-resistant and defective in the general protein transport system. The mutation also conferred additional phenotypic changes, including an inability to grow on minimal media or at 40°C. The existence of protein secretion mutants with a selectable phenotype may be useful for the genetic study of protein export. |
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Keywords: | Azide Protein secretion Protein export Acinetobacter calcoaceticus |
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