Alteration of a DNA-dependent ATPase activity in xeroderma pigmentosum complementation group C cells. |
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| Authors: | J Yanagisawa M Seki M Ui T Enomoto |
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| Affiliation: | Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan. |
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| Abstract: | DNA-dependent ATPase activities in crude extracts prepared from HeLa cells were separated into five peaks by fast protein liquid chromatography Mono Q column chromatography. Similar elution profiles were observed with the extracts from human cells normal in repair and xeroderma pigmentosum cells belonging to complementation groups A through G except for group C. An alteration in elution of one of the five ATPases, designated DNA-dependent ATPase Q1, was observed with a cell line of complementation group C. This alteration was observed with all tested cell lines that belonged to group C. ATPase Q1 in HeLa cell extracts exhibited about 2-fold higher activity with ultraviolet light-irradiated DNA as compared to that with non-irradiated DNA, whereas little difference in the effects of two DNAs was observed with the ATPase activities in the extract from group C cells. |
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