Juvenile hormones radiobiosynthesised by corpora allata of adult female locusts in vitro |
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Authors: | G E Pratt S S Tobe |
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Institution: | Agricultural Research Council, Unit of Invertebrate Chemistry and Physiology, University of Sussex, Falmer, Brighton, BN1 9QJ, U.K. |
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Abstract: | When corpora allata from adult female are incubated with either 3H-, farnesenic acid or 3H-, , bishomo-farnesenic acid and -14C] methionine, they fabricate large quantities of the corresponding double labelled methyl 10, 11-epoxy esters. Radio GLC of these products indicates retention of geometric configuration at the C-2 and C-6 double bonds. Separate analyses of the contents of the glands and medium after incubation show that the epoxy esters are rapidly released from the glands into the medium and that only the glands contain the corresponding unepoxidized esters. We suggest that unepoxidized esters are the intracellular intermediates in the formation of juvenile hormones from the unsaturated acids. Gel filtration shows that the epoxy esters are not released as stable protein complexes but as simple solutes into the medium. Using this method of promoting the synthesis of juvenile-hormone-active compounds, rates of biosynthesis of epoxy esters of up to 33 ng. per pair of glands per hour have been achieved. |
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