A nonconjugative mobilizable broad host range plasmid of Acinetobacter sp. that determines HgCl2 resistance |
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Authors: | O L Lomovskaya S Z Mindlin Zh M Gorlenko and R B Khesin |
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Institution: | (1) Institute of Molecular Genetics, USSR Academy of Sciences, Moscow, USSR |
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Abstract: | Summary HgCl2-resistant strains of Acinetobacter sp. obtained from the soil at the Khaidarkan mercury mine (Kirghiz SSR) were found to contain, apart from large plasmids ( 60 kb), a small plasmid ( 7.5 kb) designated pKL1. It was established by conjugative crosses and transformation that pKL1 is a broad host range mobilizable plasmid and that it carries the Hgr determinant. The restriction map of pKL1 was constructed; the site of the Hgr determinant and the regions essential to replication were localized. A comparison of these results with earlier data suggests that microorganisms belonging to one microbiocenosis may carry Hgr determinants on plasmids with highly different structures and properties.Deceased on July 16, 1985 |
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Keywords: | Acinetobacter HgCl2 resistance pKL1 Conjugational transfer Replication |
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