Molecular analysis distinguishes two HLA-DR3-bearing major histocompatibility complex extended haplotypes |
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Authors: | Mark Z Wescott Z L Awdeh Edmond J Yunis Dr Chester A Alper |
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Institution: | (1) The Center for Blood Research, Boston, Massachusetts, USA;(2) Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA;(3) Dana-Farber Cancer Institute, Boston, Massachusetts, USA;(4) Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA;(5) Department of Medicine, The Children's Hospital, Boston, Massachusetts, USA;(6) The Center for Blood Research, 800 Huntington Avenue, 02115 Boston, MA, USA |
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Abstract: | We detected restriction fragment length polymorphisms that distinguish the extended haplotype HLA-B8,DR3,SCO1 from HLA-B18,DR3,F1C30 at the DR beta and DQ beta loci with five of seven restriction endonucleases used. One set of restriction fragments was always found on HLA-B8,DR3,SCO1 and associated with DRw52a, while the other was present on HLA-B18, DR3,F1C30 and correlated with DRw52b (the gene encoding the subtype of DRw52 associated with the BO1 or LB-Q1 antigen). Furthermore, using a full-length DQ beta gene probe, we found division in the DQw2 haplotype, in which DQw2a always associated with HLA-B8, DR3,SCO1, while DQw2b always occurred with HLA-B18,DR3,F1C3O. Our evidence thus indicates that serologically defined HLA-DR3, HLA-DRw52, and HLA-DQw2 are each produced by two structurally very different sets of genes, one set occurring in HLA-B8, DR3,SCO1, and the other in HLA-B18,DR3,F1C30.Abbreviations used in this paper BSA
bovine serum albumin
- MHC
major histocompatibility complex
- EDTA
ethylenediaminetetraacetic acid
- SDS
sodium dodecyl sulfate |
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