PHYSIOLOGICAL AND BIOCHEMICAL CHANGES OF CARROT ROOT CALLUS DURING SUCCESSIVE CULTURES |
| |
Authors: | SYONO KUNIHIKO |
| |
Institution: | Institute of Biology, College of General Education, University of Tokyo Komaba, Meguro, Tokyo |
| |
Abstract: | - The longer the period of stock culture, the more remarkableis the growth inhibition by 8-azaguanine in callus.
- Chloramphenicol,5-methyltryptophane and mitomycin C exert greaterinhibitionon growth in CCL than in CCS.
- Bud formation is inhibited bysome concentrations of chloramphenicolwithout accompanyinginhibition of the growth.
- Cell size and the contents of RNA,DNA, protein and lipid percell of CCL are greater than thoseof CCS, respectively. Thecontents per cell of RNA and lipidin "mitochondrial fraction"are higher in CCL than in CCS.
- Incorporationof guanine-8-14C into RNA of CCS occurs rapidlyin the first12 hr and slows down thereafter, but that in CCL-RNAincreasessteadily for 16 hr. This difference in rate of theincorporationafter 12 hr between CCS and CCL is principallydue to the differencein rate of the incorporation into RNAof nuclear, mitochondrialand soluble fractions.
- The rate of RNA breakdown in CCL wasnot so great as the rateof synthesis.
- 8-azaguanine (103and 104M) inhibits incorporationof guanine-8.14C intoRNA of both CCS and CCL during 14 hr,but thereafter (up to25 hr) it inhibits the incorporation intoCCL-RNA alone leavingthat into CCS-RNA unaffected.
- In CCL 5105M 8.azaguaninedoes not affect total radioactivityincorporated into bulk RNA,but inhibits incorporation intoRNA of "mitochondrial fraction".
(Received December 23, 1964; ) |
| |
Keywords: | |
本文献已被 Oxford 等数据库收录! |
|