Non-radioactive in situ hybridization for mRNA with emphasis on the use of oligodeoxynucleotide probes |
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| Authors: | D. M. Hougaard Henrik Hansen Lars-Inge Larsson |
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| Affiliation: | (1) Department of Molecular Cell Biology, Statens Serum Institut, Artillerivej 5, Building 81, DK-2300 Copenhagen S, Denmark Tel. +45 32683544; fax +45 32683883 e-mail: hougaard@biobase.dk, DK |
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| Abstract: | Synthetic oligodeoxynucleotides represent attractive tools for mRNA in situ hybridization. In the present review, guidelines for the preparation, labeling and use of such probes is discussed. Emphasis is placed on sensitivity and specificity issues. Accepted: 20 June 1997 |
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