Limited contribution of Toll-like receptor 2 and 4 to the host response to a fungal infectious pathogen, Cryptococcus neoformans |
| |
Authors: | Nakamura Kiwamu Miyagi Kazuya Koguchi Yoshinobu Kinjo Yuki Uezu Kaori Kinjo Takeshi Akamine Morikazu Fujita Jiro Kawamura Ikuo Mitsuyama Masao Adachi Yoshiyuki Ohno Naohito Takeda Kiyoshi Akira Shizuo Miyazato Akiko Kaku Mitsuo Kawakami Kazuyoshi |
| |
Institution: | Department of Medicine and Therapeutics, Control and Prevention of Infectious Diseases, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan. kawakami@mail.tains.tohoku.ac.jp |
| |
Abstract: | The present study was designed to elucidate the role of Toll-like receptor (TLR) 2 and TLR4 in the host response to Cryptococcus neoformans. Both TLR2 knockout (KO) and TLR4KO mice produced interleukin-1beta (IL-1beta), IL-6, IL-12p40 and tumor necrosis factor-alpha (TNF-alpha) in sera and cleared this fungal pathogen from infected lungs at a comparable level to control littermate (LM) mice. Synthesis of these cytokines was not significantly different in the lungs of these KO mice and LM mice, although IL-1beta, IL-6 and IL-12p40 tended to be lower in TLR2KO, but not TLR4KO, mice than in controls. In addition, there was no significant reduction detected in the synthesis of IL-12 and TNF-alpha by bone marrow-derived dendritic cells from TLR2KO and TLR4KO mice upon stimulation with live yeast cells. Finally, HEK293 cells expressing either TLR2/dectin-1 or TLR4/MD2/CD14 did not respond to C. neoformans in the activation of nuclear factor kappa B (NFkappaB) detected by a luciferase assay. Our results suggest that TLR2 and TLR4 do not or only marginally contribute to the host and cellular response to this pathogen. |
| |
Keywords: | Cryptococcus neoformans host response TLR2 TLR4 dectin-1 |
本文献已被 PubMed 等数据库收录! |
|