RNA interference: a mammalian SID-1 homologue enhances siRNA uptake and gene silencing efficacy in human cells |
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Authors: | Duxbury Mark S Ashley Stanley W Whang Edward E |
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Institution: | Department of Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. |
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Abstract: | SID-1 is a transmembrane protein that mediates systemic RNA interference in Caenorhabditis elegans. Here we show that the mammalian SID-1 homologue FLJ20174 localizes to the cell membrane of human cells and enhances their uptake of small interfering RNA (siRNA), resulting in increased siRNA-mediated gene silencing efficacy. This is the first demonstration to show that overexpression of a membrane protein enhances siRNA internalization in mammalian cells. This observation raises the possibility of enhancing the efficacy of RNA interference. |
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Keywords: | Pancreatic Adenocarcinoma Cancer RNA interference RNAi Small interfering RNA siRNA Efficiency FLJ20174 SID-1 |
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